Recombinant Human SNCA Protein

SKU: orb2994581

Description

Recombinant Human Alpha-Synuclein is produced by our E.coli expression system and the target gene encoding Met1-Ala140 (Ala30Pro,Ala53Thr)is expressed with a 6His tag at the N-terminus.

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human SNCA (N-6His)(Cat#orb2994581) at 2μg/ml (100 μl/well) can bind Anti-Human SNCA antibody.The ED50 of Anti-SNCA antibody is 4.27ng/ml.(Regularly tested)
• Tag: N-6His
• Molecular Weight: Predicted: 16.7 KDa. Observed: 18 KDa, reducing conditions
• Expression Region: Met1-Ala140
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-Synuclein; Non-A Beta Component of AD Amyloid; Non-A4 Component of Amyloid Precursor; NACP; SNCA; NACP; PARK1

Quick Database Links

UniProt

P37840