Recombinant DIG (DIG45) antibody

• Catalog Number: orb692670
• Category: Antibodies
• Description: Recombinant DIG (DIG45) antibody
• Clonality: Recombinant
• Clone Number: DIG45
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2000, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-DIG (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against DIG-labeled probes and proteins.
• Conjugation: Unconjugated
• Source: E. coli
• Storage: Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: anti-Digoxigenin, Digoxigenin (DIG), 1672-46-4, La
• Note: For research use only
• Application notes: Anti-DIG is a his-tagged monoclonal recombinant antibody designed to detect digoxigenin and its conjugates. This antibody has been tested by ELISA, SDS-PAGE, and western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-DIG can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of DIG-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.
• Expiration Date: 12 months from date of receipt.