Digoxigenin Antibody

SKU: orb692671

Description

Recombinant DIG (DIG44) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1000, WB: 1:1000
• Reactivity: Bacteria
• Application Notes:
  Anti-DIG is a his-tagged monoclonal recombinant antibody designed to detect digoxigenin and its conjugates. This antibody has been tested by ELISA, SDS-PAGE, and western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-DIG can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of DIG-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Recombinant
• Clone No.: DIG44
• Target: Digoxigenin
• Source/Expression System: E. coli
• Purity: Anti-DIG (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against DIG-labeled probes and proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 20% (v/v) Glycerol; Buffer: 1X PBS, pH 7.4
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

anti-Digoxigenin, Digoxigenin (DIG), 1672-46-4, Lanadigenin, Digoxigenine, UNII-NQ1SX9LNAU, DIG, CHEBI:42098, HSDB 7108, NQ1SX9LNAU, EINECS 216-806-2, BRN 0096479, 4-(3,12,14-Trihydroxy-10,13-Dimethyl-Hexadecahydro-Cyclopenta[a]Phenanthren-17-Yl)-5h-Furan-2-One

ELISA Results of purified recombinant Anti-DIG Single Domain Antibody reactive against BSA-conjugated DIG. The working dilution for clone DIG44 is 1:1000. Each well was coated in duplicate with 1.0 µg BSA-conjugated DIG (Red) and BSA only (Green). The starting dilution of antibody was 45 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody (p/n orb344539) and TMB substrate (p/n orb348651).

SDS-PAGE Results of Recombinant Anti-DIG (VhH) Single Domain Antibody. Lane 1: Anti-DIG-VhH (Clone DIG44) Reduced (1 µg). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Anti-DIG-VhH (Clone DIG44) Non-Reduced (1 µg). 4-20% Gel Coomassie Blue stained. Predicted MW: ~15kDa. Observed MW: ~18kDa Reduced, ~16kDa Non-Reduced.

Western Blot of Anti-DIG Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight Marker (5 µl). Lane 2: DIG-BSA conjugated (0.2 µg). Lane 3: BSA only (0.2 µg). Lane 4: HEK293T lysate (20 µg). Left Blot - Primary Antibody: Anti-DIG (VhH) at 1 µg/ml incubated overnight at 4°C. Right Blot - No Primary. Secondary Only Control. Secondary Antibody: Mouse Anti-6X HIS DyLight™649 at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer (p/n orb348637) for 2hrs at RT. Expected MW: ~80kDa in DIG + BSA. Observed MW: (arrow) ~80kDa in DIG + BSA.

Quick Database Links

Gene Symbol

Digoxigenin