Recombinant CD47 / IAP (Integrin Associated Protein) Antibody

• Catalog Number: orb751426
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD47
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: CD47/6362R
• Tested applications: ELISA, IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: 200 μg/ml
• Dilution range: ELISA (For coating, order antibody without BSA); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: ~50kDa
• Target: CD47
• Entrez: 961
• UniProt ID: Q08722
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Antigenic Surface Determinant Protein OA3 ant
• Note: For research use only
• Application notes: Positive Control: Human placenta, brain or ovarian tumors (IHC).
• Expiration Date: 12 months from date of receipt.