CD47 Antibody

SKU: orb181434

Description

Goat polyclonal antibody to CD47.

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  This antibody is expected to recognize both reported isoforms

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CD47
• Molecular Weight: 35.2; 33.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

CD47 antibody, CD47 molecule antibody, IAP antibody, MER6 antibody, OA3 antibody, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody, CD47 glycoprotein antibody, Rh-related antigen antibody, antigen identified by monoclonal antibody 1D8 antibody, antigenic surface determinant protein OA3 antibody, integrin associ antibody

Western blot analysis of Human Hippocampus (Lane 1) and HeLa (Lane 2) lysates using CD47 antibody

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane, cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

7 µg/mL staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Quick Database Links

Gene Symbol

CD47

NCBI Gene ID

961