Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

• Catalog Number: orb607603
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD31 / PECAM-1 (Endothelial Cell Marker)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rC31.3
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant full-length human CD31 protein
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: ~100kDa (endothelium) and ~130kDa (platelets)'
• Target: PECAM-1
• Entrez: 5175
• UniProt ID: P16284
• Expression System: HEK293 cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: EndoCAM; PECA1; Platelet Endothelial Cell Adhesion
• Note: For research use only
• Application notes: Positive Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.
• Expiration Date: 12 months from date of receipt.