Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

• Catalog Number: orb1410359
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD31
• Target: PECAM-1
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Immunogen: Recombinant fragment (aa 28-330) of human CD31 expressed in E. Coli (exact sequence is proprietary)
• UniProt ID: P16284
• MW: ~100kDa (endothelium) and ~130kDa (platelets)
• Tested applications: IHC
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: Human tonsil or angiosarcoma
• Antibody Type: Recombinant Antibody
• Clone Number: C31/6446R
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EndoCAM; PECA1; Platelet Endothelial Cell Adhesion
• Note: For research use only
• Entrez: 5175