Recombinant CD3 epsilon Antibody

• Catalog Number: orb2635693
• Category: Antibodies
• Description: Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains: epsilon, gamma, delta and zeta. The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR). CD3 antigen appears at all stages of T cell development which makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 100-200 was used as the immunogen for the recombinant CD3 epsilon antibody.
• UniProt ID: P07766
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the recombinant CD3 epsilon antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: C3e/4653R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Jurkat cell lysate using recombinant CD3 epsilon antibody (clone C3e/4653R). Predicted molecular weight ~23 kDa.

IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone CD3e/4653R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant CD3 epsilon antibody (clone C3e/4653R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.