Rabbit anti-CD3E Recombinant Monoclonal Antibody

• Catalog Number: orb1519964
• Category: Antibodies
• Description: CD3E Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BL-298-5D12
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 157 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Form/Appearance: Whole IgG
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: CD3E
• UniProt ID: P07766
• NCBI: NP_000724.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CD3-epsilon; TCRE; T-cell surface glycoprotein CD3
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human CD3E by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, K-562, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH

Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human CD3E by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:250. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG

Detection of human CD3E (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: Opal™. Counterstain: DAPI (blue)

Detection of human CD3E by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CD3E recombinant monoclonal antibody (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG