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Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−
Item 1 of 6
| Tested Applications | FC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 - 1:500, Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cell. mIF: 1:250 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BL-298-5D12 |
| Immunogen | Between 157 and C-terminus |
| Target | CD3E |
| Purification | Purified |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
1 ResultsDilution
Sample
Summary
IHC
Human
1:250
Ormalin-fixed paraffin-embedded (FFPE) normal intestinal tissue and immune-related enterocolitis (irEC) tissue biopsies
The antibody was used on human...
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD antigen CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; IMD18; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000724.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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