Recombinant Bcl2 Antibody

SKU: orb2640495

Description

Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Bcl2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rBCL2/782
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant Bcl2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of human follicular lymphoma with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Bcl2 antibody (clone rBCL2/782) as confirmation of integrity and purity.

IHC testing of human tonsil tissue with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P10415