Bcl-2 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563019
• Category: Antibodies
• Description: Bcl-2 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: B9E6
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: BCL2
• UniProt ID: P10415
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Apoptosis regulator Bcl 2; Apoptosis regulator Bcl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with Bcl-2 monoclonal antibody, unconjugated (orb2563019) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.