Recombinant Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody

• Catalog Number: orb751400
• Category: Antibodies
• Description: Rabbit monoclonal antibody to BCL2
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: BCL2/6426R
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein
• Antibody Type: Recombinant Antibody
• Concentration: 200 μg/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 25-26kDa
• Target: BCL2
• Entrez: 596
• UniProt ID: P10415
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Apoptosis regulator Bcl-2 antibody, anti-B-ce
• Note: For research use only
• Application notes: Positive Control: Jurkat, K562 or HeLa cells. Human tonsil or follicular lymphomas.
• Expiration Date: 12 months from date of receipt.