RDH5 Antibody

SKU: orb1256020

Description

RDH5 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).
• Target: RDH5
• Molecular Weight: Observed: 38kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RDH5, short chain dehydrogenase/reductase family 9C, SDR9C5, retinol dehydrogenase 5 (11-cis/9-cis), retinol dehydrogenase 5 (11-cis and 9-cis), retinol dehydrogenase 1, RDH1, HSD17B9, FLJ97089, FLJ39337, 9-cis-retinol specific dehydrogenase, 11-cis RDH

Western blot analysis of extracts of various cell lines, using RDH5 antibody (orb1256020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of rat retina using RDH5 Polyclonal Antibody (orb1256020) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse retina using RDH5 Polyclonal Antibody (orb1256020) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

RDH5

UniProt

Q92781