RDH5 Antibody

• Catalog Number: orb1249095
• Category: Antibodies
• Description: RDH5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-RDRQSLPASNAFV
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in Mouse Eye lysates (calculated MW of 34.8kDa according to Mouse NP_598767.1). Recommended concentration: 2-4ug/ml.Immunohistochemistry:In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration, 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RDH5
• UniProt ID: Q92781
• NCBI: NP_002896.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RDH5, retinol dehydrogenase 5 (11-cis/9-cis), FLJ3
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in Mouse Eye lysates (calculated MW of 34.8kDa according to Mouse NP_598767.1). Recommended concentration: 2-4ug/ml.Immunohistochemistry:In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249095 (2 ug/ml) staining of Mouse Eye lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249095 (5 ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.