RDH5 Antibody

SKU: orb1249095

Description

RDH5 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in Mouse Eye lysates (calculated MW of 34.8kDa according to Mouse NP_598767.1). Recommended concentration: 2-4ug/ml.Immunohistochemistry:In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration, 5-10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RDRQSLPASNAFV
• Target: RDH5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

RDH5, retinol dehydrogenase 5 (11-cis/9-cis), FLJ39337, FLJ97089, HSD17B9, RDH1, SDR9C5, 11-cis RDH, 9-cis-retinol specific dehydrogenase, retinol dehydrogenase 1, retinol dehydrogenase 5 (11-cis and 9-cis), short chain dehydrogenase/reductase family 9C, member 5

orb1249095 (2 ug/ml) staining of Mouse Eye lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249095 (5 ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RDH5

UniProt

Q92781

RefSeq (Protein):NP_002896.2