RBM20 Antibody

• Catalog Number: orb1249015
• Category: Antibodies
• Description: RBM20 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-QLDKAELDERP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 150kDa band observed in lysates of cell line HEK293 (calculated MW of 134kDa according to Human NP_001127835.2). Preliminary experiments also showed a 150kDa band in lU2OS cell lysate and in Human Smooth Muscle lysate.. The observed molecular weight corresponds to different antibodies from other commercial sources..Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RBM20
• UniProt ID: Q5T481
• NCBI: NP_001127835.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RBM20, RNA binding motif protein 20
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 150kDa band observed in lysates of cell line HEK293 (calculated MW of 134kDa according to Human NP_001127835.2). Preliminary experiments also showed a 150kDa band in lU2OS cell lysate and in Human Smooth Muscle lysate.. The observed molecular weight corresponds to different antibodies from other commercial sources..Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
• Expiration Date: 12 months from date of receipt.

orb1249015 (0.3 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer) Detected by chemiluminescence.