RBM20 (aa717-727) Antibody

SKU: orb125077

Description

Goat Polyclonal to RBM20. NP_001127835.1, PMID: 20590677 and GeneID: 282996.

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  WB: Approx 150kDa band observed in lysates of cell line HEK293 (calculated MW of 134kDa according to Human NP_001127835.2). Preliminary experiments also showed a 150kDa band in lU2OS cell lysate and in Human Smooth Muscle lysate. This molecular weight is rout

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RBM20 (aa717-727)
• Protein Sequence: QLDKAELDERP
• Molecular Weight: 134
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RBM20 antibody, anti RNA binding motif protein 20 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nucleoli and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

RBM20 (aa717-727)

NCBI Gene ID

282996