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Goat anti-RBM20 (aa717-727) Antibody
Catalog Number: orb125077
| Catalog Number | orb125077 |
|---|---|
| Category | Antibodies |
| Description | Goat Polyclonal to RBM20. NP_001127835.1, PMID: 20590677 and GeneID: 282996. |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF |
| Reactivity | Bovine, Human, Mouse, Rat |
| Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml |
| Conjugation | Unconjugated |
| MW | 134 |
| Target | RBM20 (aa717-727) |
| Entrez | 282996 |
| Protein Sequence | QLDKAELDERP |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti RBM20 antibody, anti RNA binding motif protei Read more... |
| Note | For research use only |
| Application notes | WB: Approx 150kDa band observed in lysates of cell line HEK293 (calculated MW of 134kDa according to Human NP_001127835.2). Preliminary experiments also showed a 150kDa band in lU2OS cell lysate and in Human Smooth Muscle lysate. This molecular weight is rout |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nucleoli and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
