Rat IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb347889

Description

Rat IgG (H&L) antibody (FITC)

Images & Validation

• Tested Applications: FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Rat
• Application Notes:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Donkey
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rat IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 2.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Donkey Anti-Rat IgG fluorescein Conjugated Antibody, Donkey Anti-Rat IgG Antibody FITC Conjugation

Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n orb347888). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n orb347888) 1:10000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347994) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.