F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348342
• Category: Antibodies
• Description: F(ab')2 Rat IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: FITC
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: 0.5 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
• Immunogen: Rat IgG whole molecule
• Tested applications: FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: F(ab')2 Anti-Rat IgG (H&L) Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat F(ab')2 Anti-Rat IgG Fluorescein Conjugated A
• Note: For research use only

Western Blot of Goat anti-F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed Lane 1: Rat IgG Load: 50 ng per lane Secondary antibody: F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed at 1:1000 for 60 min at RT Block: orb348637 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa