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Catalog Number | orb1247904 |
---|---|
Category | Antibodies |
Description | RAN Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: AAQGEPQVQFKLV-C |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in lysates of cell lines A431, HeLa, HepG2 and NIH3T3 (calculated MW of 24.4kDa according to NP_006316.1). Recommended for use at 0.3-1ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | RAN |
UniProt ID | P62826 |
NCBI | NP_006316.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | RAN, TC4, ARA24, RAN, member RAS oncogene family, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in lysates of cell lines A431, HeLa, HepG2 and NIH3T3 (calculated MW of 24.4kDa according to NP_006316.1). Recommended for use at 0.3-1ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247904 (0.5 ug/ml) staining of A431 (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1247904 (3.75 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data was obtained using a previous batch.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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