RAD18 Antibody

• Catalog Number: orb1536789
• Category: Antibodies
• Description: RAD18 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3H7
• Tested applications: ELISA, IF, IHC, IHC-P, RNA-I, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b,k
• Immunogen: RAD18 (NP_064550, 332 aa ~ 431 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Concentration: 0.5 mg/ml
• Dilution range: ELISA (1:100 - 1:2000), IF (25 µg/ml), IHC, IHC-P (1.5 - 10 µg/ml), RNAi, WB (1:500)
• Conjugation: Unconjugated
• Target: RAD18
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Alternative names: RAD18, HHR18, HRAD18, RNF73, RAD18 homolog (S. cer
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
• Expiration Date: 12 months from date of receipt.

Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]

Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell. [antibody concentration 25 ug/ml]

Western blot of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.

Detection limit for recombinant GST tagged RAD18 is approximately 0.1 ng/ml as a capture antibody.

Western blot of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody, clone 3H7. GAPDH (36.1 kD) used as specificity.