PCGF4/BMI1 Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb7539
• Category: Antibodies
• Description: PCGF4/BMI1 Rabbit Polyclonal Antibody (FITC)
• Target: BMI1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Rabbit, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Bmi1/PCGF4 (61-160/326aa)
• RRID: AB_10929779
• Tested applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: B lymphoma Mo MLV insertion region; Bmi 1; BMI-1;
• Note: For research use only

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-PCGF4 BMI1 antibody (orb7539), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control (blue line): Mouse kidney (Black). Primary Antibody (green line): Rabbit Anti-PCGF4 BMI1/FITC antibody (orb7539), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (5 min at -20°C) and then permeabilized with ice-cold mehtanol for 20 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-PCGF4/BMI1 Antibody, conjugated (orb7539) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.