PCGF4/BMI1 Rabbit Polyclonal Antibody (FITC)

SKU: orb7539

Description

PCGF4/BMI1 Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting BMI1. This antibody is suitable for FC. It exhibits reactivity with Human, Mouse samples.

Research Area

Cancer Research, Cell Biology, Cell Differentiation, Epigenetics, Polycomb Repressive Complex 2 (PRC2), Proto-oncogenes

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bmi1/PCGF4 (61-160/326aa)
• Target: BMI1
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

B lymphoma Mo MLV insertion region; Bmi 1; BMI-1; MGC12685; Murine leukemia viral(bmi 1) oncogene homolog; Oncogene BMI 1; PCGF 4; PCGF4; Polycomb complex protein BMI 1; Polycomb complex protein BMI-1; Polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; BMI1; RNF51; RAD18; BMI1_HUMAN.

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-PCGF4 BMI1 antibody (orb7539), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control (blue line): Mouse kidney (Black). Primary Antibody (green line): Rabbit Anti-PCGF4 BMI1/FITC antibody (orb7539), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (5 min at -20°C) and then permeabilized with ice-cold mehtanol for 20 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-PCGF4/BMI1 Antibody, conjugated (orb7539) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

BMI1