RAC1 Antibody

• Catalog Number: orb1927136
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: P63000
• MW: 21450 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:100
• Clone Number: 1301CT276.121.104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ras-related C3 botulinum toxin substrate 1, Cell m
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded H.skin section using RAC1. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using RAC1. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of U-87 MG cells using RAC1 (green) compared to an isotype control of mouse IgG2b (blue). Diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from A431, mouse NIH/3T3, rat C6 cell line (from left to right), using RAC1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.