RAC1 Antibody

SKU: orb1927136

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:25, FC - 1:100
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: B3523EV498X343X326
• Immunogen: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Target: RAC1 (HGNC:9801)
• Molecular Weight: 21450 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, RAC1, TC25

Immunohistochemical analysis of paraffin-embedded H.skin section using RAC1. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using RAC1. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of U-87 MG cells using RAC1 (green) compared to an isotype control of mouse IgG2b (blue). Diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from A431, mouse NIH/3T3, rat C6 cell line (from left to right), using RAC1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Quick Database Links

Gene Symbol

RAC1 (HGNC:9801)

UniProt

P63000