Phospho-PAK4 (Ser99) Rabbit Polyclonal Antibody

• Catalog Number: orb5742
• Category: Antibodies
• Description: Phospho-PAK4 (Ser99) Rabbit Polyclonal Antibody
• Target: PAK4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rabbit
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99 SN(p-S)LR
• UniProt ID: O96013
• RRID: AB_10928146
• MW: 64 kDa
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: p21 activated kinase 4; p21(CDKN1A) activated kina
• Research Area: Cell Biology, Kinases, Phosphatases
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): NIH/3T3. Primary Antibody (green line): Rabbit Anti-Phospho-PAK4 (Ser99) antibody (orb5742), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

phosphopeptide, non phosphopeptide.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-PAK4 (Ser99) (orb5742) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-PAK4 (Ser99) (orb5742) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.

Tissue/Cell: human prostate tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PAK4 (Ser99) Polyclonal Antibody, Unconjugated (orb5742) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.