Rabbit IgG (H+L) (GOAT) Antibody Peroxidase Conjugated

SKU: orb3145400

Description

Rabbit IgG (H+L) (GOAT) Antibody Peroxidase Conjugated

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: User Optimized Western Blot 1:10,000 ELISA 1:20,000 – 1:40,000 Immunohistochemistry 1:500 – 1:1,000 (Other Assays User Optimized)
• Reactivity: Rabbit
• Application Notes:
  This anti-rabbit HRP secondary antibody is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Rabbit IgG whole molecule
• Purification: Affinity column purified
• Conjugation: HRP

Storage & Handling

• Storage: Long time storage is recommended at -20°C
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (rAlbumin), 50% Glycerol, 0.025% ProClin 300 was added as a preservative, Do NOT add Sodium Azide!
• Concentration: 1 mg/ml by UV absorbance at 280 nm
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western Blot of Peroxidase Conjugated Goat Anti-Rabbit IgG Antibody. Lysates/proteins at 35 μg per lane. The membrane was then blocked for an hour before being incubated with IR3-8 GAPDH antibody overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit IgG Antibody (orb3145400) at 1:10,000 for 60 min at RT. Predicted/Observed size: 35 kDa.