AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated

SKU: orb608184

Description

AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated

Images & Validation

• Tested Applications: ELISA, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:5000-20000, IHC-P=1:3000-5000, IHC-F=1:3000-5000, ELISA=1:5000-20000
• Reactivity: Rabbit

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Native Rabbit IgG
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP; AffiniPure Goat Anti-Rabbit IgG H&L/HRP; AffiniPure Goat Anti-Rabbit IgG H&L(HRP); HRP AffiniPure Goat Anti-Rabbit IgG (H+L); Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (orb608184) at 1/200 dilution and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (orb608184) at 1/200 dilution and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (orb608184) at 1/200 dilution and DAB staining.