Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb347680

Description

Rabbit IgG F(ab')2 antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:130,000, IHC: 1:1,000 - 1:10,000, WB: 1:2,000 - 1:20,000
• Reactivity: Rabbit
• Application Notes:
  Anti-Rabbit IgG F(ab')2 fragment has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG F(ab')2 fragment in goat.
• Purity: Anti-RABBIT IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat Anti-Rabbit IgG F(ab')2 Antibody peroxidase Conjugation, Goat Anti-Rabbit IgG Fab2 peroxidase Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment HRP Conjugated Antibody

Western Blot of Anti-Rabbit IgG F(ab')2 (GOAT) Antibody (p/n orb535108). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab')2 (GOAT) Antibody (p/n orb535108) 1:10000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.