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Rabbit Vinculin Antibody
Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-Vinculin antibody A302-535A. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Gallus, Porcine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 750 and 800 |
| Target | Vinculin |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human and mouse Vinculin by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Vinculin antibody orb1526458 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003364.1 |
|---|
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Rabbit Vinculin Antibody (orb1526458)
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