Rabbit Vinculin Antibody

SKU: orb1526456

Description

Vinculin Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Porcine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1084 and C-terminus
• Target: Vinculin
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin

Detection of human Vinculin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, PC-3, HEK293T, and OVCAR-4 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Vinculin antibody (orb1526456) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse Vinculin by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Vinculin antibody (orb1526456) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Vinculin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from PC-3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Vinculin antibody (orb1526456) used for IP at 6 µg per reaction.

Detection of human Vinculin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Vinculin (Cat. No. orb1526456) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Vinculin by immunohistochemistry.Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti- Vinculin (Cat. No. orb1526456) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Vinculin

UniProt

P18206

RefSeq (Protein):NP_003364.1