Rabbit TRIM25 Antibody

SKU: orb1527667

Description

TRIM25 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 400 and 450
• Target: TRIM25
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin/ISG15 ligase TRIM25; EFP; estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; RNF147; tripartite motif protein TRIM25; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; Z147; Zinc finger protein 147; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147; ZNF147

Detection of human TRIM25 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TRIM25 antibody orb1527667 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. TRIM25 was also immunoprecipitated by rabbit anti-TRIM25 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Quick Database Links

Gene Symbol

TRIM25

UniProt

Q14258

RefSeq (Protein):NP_005073.2