Rabbit TRIM25 Antibody

SKU: orb1527669

Description

TRIM25 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 350 and 400
• Target: TRIM25
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin/ISG15 ligase TRIM25; EFP; estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; RNF147; tripartite motif protein TRIM25; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; Z147; Zinc finger protein 147; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147; ZNF147

Detection of human TRIM25 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, LNCaP, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIM25 antibody orb1527669 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human TRIM25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIM25 antibody orb1527669 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

TRIM25

UniProt

Q14258

RefSeq (Protein):NP_005073.2