Rabbit TOPORS Antibody

SKU: orb1527074

Description

TOPORS Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 995 and C-terminus
• Target: TOPORS
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase Topors; LUN; p53-binding protein 3; P53BP3; RING-type E3 ubiquitin transferase Topors; RP31; SUMO1-protein E3 ligase Topors; topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase; Topoisomerase I-binding arginine/serine-rich protein; topoisomerase I-binding RING finger protein; TP53BPL; tumor suppressor p53-binding protein 3

Detection of human TOPORS by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TOPORS antibody orb1527074 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. TOPORS was also immunoprecipitated by rabbit anti-TOPORS antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 minute (A) and 30 seconds (B).

Detection of human TOPORS by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-TOPORS (Cat. No. orb1527074) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

TOPORS

UniProt

Q9NS56

RefSeq (Protein):NP_005793.2