Rabbit SET Antibody

SKU: orb1526942

Description

SET Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 150 and 200
• Target: SET
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2PP2A; chromatin remodelling factor; HLA-DR-associated protein II; I2PP2A; I-2PP2A; IGAAD; inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; IPP2A2; MRD58; PHAPII; phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; protein SET; SET nuclear oncogene; SET translocation (myeloid leukemia-associated); TAF-I; TAF-IBETA; Template-activating factor I; Template-Activating Factor-I, chromatin remodelling factor

Detection of mouse SET by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody orb1526942 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human SET by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, HeLa, Jurkat, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody orb1526942 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SET by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SET antibody orb1526942 used for IP at 6 µg per reaction.

Detection of mouse SET by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. orb1526942) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of human SET by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. orb1526942) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SET

UniProt

Q01105

RefSeq (Protein):NP_001116293.1