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Catalog Number | orb11565 |
---|---|
Category | Antibodies |
Description | VSIG4 Rabbit Polyclonal Antibody |
Target | VSIG4 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Mouse, Rat |
Predicted Reactivity | Bovine, Canine, Human |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide derived from human VSIG4 (81-160/399aa) |
UniProt ID | Q9Y279 |
RRID | AB_10753286 |
MW | 33 kDa |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | V-set and immunoglobulin domain-containing protein Read more... |
Note | For research use only |
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Yuan Liao, Chaonan Deng, Xichuan Wang VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis Tissue Cell, 86, 102285 (2024)
Applications
Reactivity
Sample: Lung (Mouse) Lysate at 30 ug, Placenta (Mouse) Lysate at 30 ug, Primary: Anti-VSIG4 (orb11565) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.
Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-VSIG4 (orb11565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.
Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-VSIG4 (orb11565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.
Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VSIG4 Polyclonal Antibody, Unconjugated (orb11565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |