VSIG4 Rabbit Polyclonal Antibody

• Catalog Number: orb11565
• Category: Antibodies
• Description: VSIG4 Rabbit Polyclonal Antibody
• Target: VSIG4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human VSIG4 (81-160/399aa)
• UniProt ID: Q9Y279
• RRID: AB_10753286
• MW: 33 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: V-set and immunoglobulin domain-containing protein
• Research Area: Inflammation, Macrophage
• Note: For research use only

References

Yuan Liao, Chaonan Deng, Xichuan Wang VSIG4 ameliorates intestinal inflammation through inhibiting macrophages NLRP3 inflammasome and pyroptosis Tissue Cell, 86, 102285 (2024)

PubMed: 38113649

Applications

IHC

Reactivity

Human

Sample: Lung (Mouse) Lysate at 30 ug, Placenta (Mouse) Lysate at 30 ug, Primary: Anti-VSIG4 (orb11565) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-VSIG4 (orb11565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-VSIG4 (orb11565) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VSIG4 Polyclonal Antibody, Unconjugated (orb11565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.