Rabbit RPL5 Antibody

SKU: orb1523907

Description

RPL5 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rabbit, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 247 and C-terminus
• Target: RPL5
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60S ribosomal protein L5; L5; large ribosomal subunit protein uL18; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; uL18

Detection of human and mouse RPL5 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL5 antibody orb1523907 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RPL5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody orb1523907 used for IP at 3 µg per reaction.

Detection of human RPL5 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. orb1523907) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human RPL5 by immunohistochemistry.Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. orb1523907) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RPL5

UniProt

P46777

RefSeq (Protein):NP_000960.2