Rabbit RPA32 Antibody

SKU: orb1530481

Description

RPA32 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 220 and C-terminus
• Target: RPA32
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32

Detection of human RPA32 by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, and U2OS cells. Antibody: Affinity purified rabbit anti-RPA32 antibody at 0.1 µg/ml.

Detection of mouse RPA32 by western blot.Samples: Whole cell lysate from CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-RPA32 antibody at 0.04 µg/ml.

Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5, 000 (0.2 µg/ml).

Detection of mouse RPA32 by immunohistochemistry. Sample: FFPE section of mouse kidney. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5, 000 (0.2 µg/ml).

Detection of human RPA32 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody.

Quick Database Links

Gene Symbol

RPA32

UniProt

P15927

RefSeq (Protein):NP_002937.1