Rabbit Phospho RPA32 (S4/S8) Antibody

SKU: orb1530479

Description

RPA32, Phospho (S4/S8) Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Surrounding serine 4/8
• Target: RPA32 Phospho (S4/S8)
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32

Detection of human Phospho RPA32 (S4/S8) by western blot.Samples: Whole cell lysate from BeWo and AlphaTC1 Clone 9 cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody at 0.1 µg/ml.

Detection of human Phospho RPA32 (S4/S8) by IHC. Sample: FFPE section of human breast carcinoma, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1, 000.

Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse gut, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1, 000.

Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells treated with or without etoposide. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody.

Quick Database Links

Gene Symbol

RPA32 Phospho (S4/S8)

UniProt

P15927

RefSeq (Protein):NP_002937.1