Rabbit RNA Polymerase II, Phospho (S2) Antibody

SKU: orb1523052

Description

RNA Polymerase II, Phospho (S2) Antibody

Research Area

Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Surrounding serine 2
• Target: Phospho RNA Polymerase II (S2)
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: RNA Polymerase II was immunoprecipitated using rabbit anti-RNA Polymerase II antibody. Following immunoprecipitation the product of the IP reaction was mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1523052 was used for WB at 1 µg/ml (upper panel). To examine total RNA Polymerase II, the blot was stripped and then blotted with rabbit anti-RNA Polymerase II antibody (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper panel) and 15 seconds (lower panel).

Detection of human Phospho RNA Polymerase II (S2) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) (Cat. No. orb1523052) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse Phospho RNA Polymerase II (S2) by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) (Cat. No. orb1523052) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1523052 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

Phospho RNA Polymerase II (S2)

UniProt

P24928

RefSeq (Protein):NP_000928.1