RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rabbit Polyclonal Antibody (BF647)

SKU: orb1579242

Description

RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rabbit Polyclonal Antibody (BF647) is a BF647 conjugated antibody targeting POLR2A. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Rat samples.

Research Area

DNA and RNA, Epigenetics and Nuclear Signaling, Polymerase, RNA Polymerases

Images & Validation

• Tested Applications: FC, IF
• Dilution range: Flow-Cyt=1ug/Test, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser2 Y(p-S)PT
• Target: POLR2A
• Purification: Affinity purified by Protein A
• Conjugation: BF647

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Rpb1 CTD; RNA polymerase II; RNAPII; POLR2A; POLR2; DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.

Blank control: HL60. Primary Antibody (green line): Rabbit Anti-RNA polymerase II CTD repeat YSPTSPS antibody Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

POLR2A