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Catalog Number | orb1529979 |
---|---|
Category | Antibodies |
Description | RIF1 Antibody |
Target | RIF1 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Whole IgG |
Concentration | 1000 µg/ml |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Immunogen | Between 1425 and 1475 |
UniProt ID | Q5UIP0 |
Tested applications | IHC, IP, WB |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Application notes | Format: Whole IgG |
Antibody Type | Primary Antibody |
Storage | 2 - 8°C |
Alternative names | telomere-associated protein RIF1; RAP1 interacting Read more... |
Note | For research use only |
NCBI | NP_060621.3 |
Expiration Date | 12 months from date of receipt. |
Detection of human Rif1 by western blot.Samples: Whole cell lysate from HeLa, K-562, HEK293T, MCF-7, and A-549 cells. Antibody: Affinity purified rabbit anti-Rif1 antibody at 0.04 µg/ml.
Detection of human RIF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human Rif1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-Rif1 antibody.