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Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 529 and C-terminus |
| Target | RelB |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human and mouse RelB by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from Jurkat (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HeLa (H; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-RelB antibody orb1527066 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. RelB was also immunoprecipitated by rabbit anti-RelB antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_006500.2 |
|---|
Documents Download
Request a Document
Protocol Information
Rabbit RelB Antibody (orb1527066)
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![NF-κB p65 Rabbit mAb [KD Validated] Antibody](/images/pub/media/catalog/product/NewWebsite/18/orb1951958_1.png)
![NF-κB p65 Rabbit mAb [KD Validated] Antibody](/images/pub/media/catalog/product/NewWebsite/18/orb1951958_2.png)
![NF-κB p65 Rabbit mAb [KD Validated] Antibody](/images/pub/media/catalog/product/NewWebsite/18/orb1951958_3.png)
![NF-κB p65 Rabbit mAb [KD Validated] Antibody](/images/pub/media/catalog/product/NewWebsite/18/orb1951958_4.png)

