Rabbit RAP74 Antibody

SKU: orb1526048

Description

RAP74 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 275 and 325
• Target: RAP74
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BTF4; general transcription factor IIF 74 kDa subunit; general transcription factor IIF subunit 1; general transcription factor IIF, polypeptide 1, 74kDa; RAP74; TF2F1; TFIIF; TFIIF-alpha; transcription initiation factor IIF subunit alpha; transcription initiation factor RAP74

Detection of human RAP74 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAP74 antibody orb1526048 used for IP at 3 µg/mg lysate.

Detection of human RAP74 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RAP74 (Cat. No. orb1526048) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RAP74

UniProt

P35269

RefSeq (Protein):NP_002087.2