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Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Catalog Number: orb1806452

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1806452
CategoryAntibodies
DescriptionRabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberBLR284L
Tested applicationsWB
ReactivityHuman, Mouse
ImmunogenBetween 225 and 275
Antibody TypePrimary Antibody
Concentration500 µg/ml
Dilution rangeWB: 1:1000
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetPVRL1/NECTIN1
UniProt IDQ15223
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Alternative namesPVRL1; nectin-1; OFC7; poliovirus receptor-like 1;
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NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (10 µg) from MCF-10A, SNB-75, HaCaT, MCF-7, and RT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1806452) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of mouse PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from ND7/23, C6, RAW 264.7, J774A1, and AML12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1806452) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.