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Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Catalog Number: orb1784567

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1784567
CategoryAntibodies
DescriptionRabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberBLR274L
Tested applicationsFC, IP, WB
ReactivityHuman
ImmunogenResidues 31-331 (ECD)
Antibody TypePrimary Antibody
Concentration1000 µg/ml
Dilution rangeWB: 1:1000, IP: 6 µl/mg lysate
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetPVRL1/NECTIN1
UniProt IDQ15223
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Alternative namesPVRL1; nectin-1; OFC7; poliovirus receptor-like 1;
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NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used for IP at 6 µl/mg lysate.