Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody

• Catalog Number: orb1784567
• Category: Antibodies
• Description: Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody
• Target: PVRL1/NECTIN1
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Immunogen: Residues 31-331 (ECD)
• UniProt ID: Q15223
• Tested applications: FC, IP, WB
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR274L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PVRL1; nectin-1; OFC7; poliovirus receptor-like 1;
• Note: For research use only

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used for IP at 6 µl/mg lysate.