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Catalog Number | orb1784567 |
---|---|
Category | Antibodies |
Description | Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR274L |
Tested applications | FC, IP, WB |
Reactivity | Human |
Immunogen | Residues 31-331 (ECD) |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1000, IP: 6 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | PVRL1/NECTIN1 |
UniProt ID | Q15223 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | PVRL1; nectin-1; OFC7; poliovirus receptor-like 1; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used for IP at 6 µl/mg lysate.
FC, IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |