Rabbit anti-Phospho KAP-1 (S824) Antibody

SKU: orb1529675

Description

KAP-1, Phospho (S824) Antibody

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:200 - 1:1,000; ICC-IF - 1:200 - 1:1,000
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Surrounding serine 824
• Target: KAP-1, Phospho (S824)

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

KRAB [Kruppel-associated box domain]-associated protein 1; RING finger protein 96; Tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; transcription intermediary factor 1-beta; TIF1-beta; TIF1B; TF1B; RNF96; RING-type E3 ubiquitin transferase TIF1-beta; protein phosphatase 1, regulatory subunit 157; PPP1R157; nuclear corepressor KAP-1; KRIP-1; KRAB-associated protein 1; KAP-1; KAP1; E3 SUMO-protein ligase TRIM28; KRAB-interacting protein 1

Detection of human phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.

Detection of mouse phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.

Detection of human Phospho KAP-1 (S824) by IHC.Samples: FFPE serial sections of prostate carcinoma with MOCK CIP treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at a dilution of 1:200.

Detection of human phospho KAP-1 (S824) by western blot of immunoprecipitates.Samples: HEK293T cells. Antibodies: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody and rabbit anti-KAP-1 recombinant monoclonal antibody.

Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells with MOCK CIP treatment (left) and with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at 1:200.

Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200. Detection: Red fluorescent anti-rabbit IgG-DyLight® 594 conjugated.

Quick Database Links

Gene Symbol

KAP-1, Phospho (S824)

UniProt

Q13263

RefSeq (Protein):NP_005753.1