Phospho-TRIM28 (Ser824) Rabbit Polyclonal Antibody

SKU: orb7084

Description

Phospho-TRIM28 (Ser824) Rabbit Polyclonal Antibody

Research Area

Apoptosis, Bromodomains (BRDs), Cell Biology, Cell Cycle, DNA and RNA, DNA and RNA Research, Methylated DNA, Transcription Factors

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TRIM28 around the phosphorylation site of Ser824 LS(p-S)QE
• Target: TRIM28
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TIF1 beta(phospho-Ser824); P-TIF1 beta(Ser824); KAP1(phospho S824); p-KAP1(phospho S824); TIF1B_HUMAN; Transcription intermediary factor 1-beta; KAP1; RNF96; TIF1B; TIF1-beta; TIF1 beta; E3 SUMO-protein ligase TRIM28; EC:2.3.2.27; KRAB-associated protein 1(KAP-1); KRAB-interacting protein 1(KRIP-1); Nuclear corepressor KAP-1; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; Tripartite motif-containing protein 28;

Molt-4 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-TIF1 beta (Ser824) Antibody (orb7084) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Quick Database Links

Gene Symbol

TRIM28

UniProt

Q13263