Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody

SKU: orb1570943

Description

PDGFR beta Antibody

Images & Validation

• Tested Applications: FC, ICC, IP, WB
• Dilution range: WB - 1:1,000; IP - 6 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
• Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR242L
• Immunogen: Residues 32-530 (ECD)
• Target: PDGFR beta

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

CD antigen CD140b; platelet-derived growth factor receptor beta variant 1; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor 1; PDGFR-beta; PDGF-R-beta; PDGFR-1; Pdgfr; PDGF beta chain; AI528809; CD140 antigen-like family member B; beta-type platelet-derived growth factor receptor; beta platelet-derived growth factor receptor; CD140b

Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 µg) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse PDGFR beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used for IP at 6 µl/mg lysate.

Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

PDGFR beta

UniProt

P05622

RefSeq (Protein):NP_032835.2