Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody

• Catalog Number: orb1570943
• Category: Antibodies
• Description: PDGFR beta Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR242L
• Tested applications: FC, ICC, IP, WB
• Reactivity: Mouse
• Immunogen: Residues 32-530 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000; IP - 6 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PDGFR beta
• UniProt ID: P05622
• NCBI: NP_032835.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: CD antigen CD140b; platelet-derived growth factor
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 µg) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse PDGFR beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used for IP at 6 µl/mg lysate.

Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.