PDGF Receptor beta Recombinant Rabbit Monoclonal Antibody

SKU: orb2563055

Description

PDGF Receptor beta Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-800, IF=1:200-800, IHC-F=1:200-800
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 3A3
• Immunogen: A synthesized peptide derived from human PDGFR beta (1051-1106aa)
• Target: PDGFRB
• Molecular Weight: 118 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Disclaimer: For research use only

Alternative Names

Platelet-derived growth factor receptor beta; CD140B; beta platelet derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD 140B; CD140 antigen-like family member B; CD140B; CD140b antigen; JTK12; OTTHUMP00000160528; PDGF R beta; PDGF-R-beta; PDGFR; PDGFR1; PDGFRB; PGFRB_HUMAN; Platelet derived growth factor receptor beta polypeptide; PDGF Receptor beta; beta-type platelet-derived growth factor receptor precursor;

25 ug total protein per lane of various lysates probed with PDGF Receptor beta monoclonal antibody, unconjugated (orb2563055) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDGF Receptor beta Monoclonal Antibody, Unconjugated (orb2563055) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDGF Receptor beta Monoclonal Antibody, Unconjugated (orb2563055) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Uterus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDGF Receptor beta Monoclonal Antibody, Unconjugated (orb2563055) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

PDGFRB

UniProt

P09619