Rabbit p53 Antibody

SKU: orb1530475

Description

p53 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ChIP, FC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 50 and 100
• Target: p53
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

antigen NY-CO-13; BCC7; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; Tumor suppressor p53; tumor supressor p53

Detection of human P53 by western blot.Samples: Whole cell lysate from HEK293T cells. Antibody: Affinity purified rabbit anti-P53 antibody orb1530475 at 0.1 µg/ml.

Detection of human p53 by immunohistochemistry. Sample: FFPE section of human lung cancer Antibody: Affinity purified rabbit anti-p53 used at 1:5, 000 (0.2µg/ml).

Detection of human P53 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-P53 antibody.

Localization of p53 Binding Sites by ChIP-sequencing. Chromatin from MCF-7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing.

Quick Database Links

Gene Symbol

p53

UniProt

P04637

RefSeq (Protein):NP_000537.3