Rabbit anti-Oct-4 Antibody

• Catalog Number: orb1522713
• Category: Antibodies
• Description: Oct-4 Antibody
• Target: Oct-4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 1 and 50
• UniProt ID: P20263
• Tested applications: IP, WB
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: Otf3; POU domain, class 5, transcription factor 1;
• Note: For research use only
• NCBI: NP_038661.1

Detection of mouse Oct-4 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Oct-4 antibody orb1522713 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse Oct-4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Oct-4 antibody orb1522713 used for IP at 6 µg per reaction.