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Description
Research Area
DNA / RNA Binding, DNA and RNA Research, Transcription Factors
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Human, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human OCT4 (201-300/360aa) |
| Target | POU5F1 |
| Molecular Weight | 47 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−OCT3; OCT4; OCT4Borf1; OTF-3; OTF3; OTF4; Oct-3; Oct-4; Oct3/4; NF-A3; Oct-3/4; Otf-4; Otf3-rs7; Otf3g; PO5F1_HUMAN; POU5F1; Octamer-binding protein 3 (Oct-3); Octamer-binding protein 4 (Oct-4); Octamer-binding transcription factor 3 (OTF-3); PO5F1_MOUSE; POU class 5 homeobox 1; POU domain class 5, transcription factor 1
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Quick Database Links
Gene Symbol
POU5F1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence






